Absorbance reader with spectrometer, Complete spectrum within 1 second
The new SPECTROstar Omega can measure user-defined fixed wavelengths or it can capture a full absorbance spectrum (200 to 850 nm) in less than 1 second per well, at a resolution of 1 nm. Combined with precise temperature control, on-board injection, shaking, high reproducibility and a wide dynamic range, the SPECTROstar Omega provides users with unmatched flexibility and sensitivity to perform any absorbance based assay needed.
Applications include
- 260/280 nm DNA quantitation
- Protein quantitation (Bradford, Lowry)
- Reporter gene assays (ß-Gal, SEAP)
- Cell proliferation and apoptosis
- ELISA, enzyme immunoassays (alkaline phosphatase, horseradish peroxidase)
- Enzyme activity assays
- Microbial growth
And many more assays
Upgrades
Due to the absolutely modular concept of the Omega reader family the SPECTROstar Omega is entirely upgradeable to a multidetection reader. Whenever the need for fluorescence and luminescence based experiments arrives, have the SPECTROstar Omega upgraded to a FLUOstar Omega or even the POLARstar Omega with simultaneous dual emission and fluorescence polarization detection mode.
Incubation
Stable and uniform incubation is achieved by temperature-regulated heating plates above and below the entire microplate movement area. The top incubation plate is at a slightly higher temperature than the bottom plate to help reduce condensation when lids or sealers are used. Incubation up to 60°C is available for users with elevated temperature needs.
On-board reagent injectors
The SPECTROstar Omega can be equipped with one or two integrated syringe injectors (no bulky add-on boxes beside or on top of the microplate reader). The injectors can deliver agonists, substrates, enzymes - even cells, for advanced microplate assays. Injection speed and timing is fully adjustable within the control software. Up to four different volumes, between 5 to 350 µL in 0.5 µL increments, can be delivered to each well; thereby allowing the user to produce dilution schemes, concentration ranges, etc.
Endpoint, slow and fast kinetic measurements
Kinetic data can be collected as fast as 50 reading points per second or as slow as one reading point every 2˝ hours. You can capture a fast calcium signal that happens in a few seconds or measure bacterial growth over a period of days. Furthermore, data can be collected at different rates within the same experiment; this allows the user to collect more data points at regions of interest (i.e. growth phase) and less data points in areas of little change (i.e. lag phase).
Stacker and robot compatibility
BMG LABTECH’s standardized reader footprint and robotic software interface make it easy to integrate the SPECTROstar Omega into all existing robotic systems. The Stacker II with 50 plates capacity and optional barcode reader, is the modular attachment that can be integrated with the SPECTROstar Omega for medium throughput plate handling.
Software
BMG LABTECH’s Omega Windows™ based software has an easy to use control package and a complete new powerful data evaluation segment. It is compatible with the new Windows Vista™ operating system and is fully compliant with FDA regulation 21 CFR Part 11.
The control software allows flexible definition of microplate layouts, filter selection, interval and injection times, as well as measurement frequency, heating, and shaking parameters. During measurement, it is possible to observe the data with the Real Time well display feature, which allows viewing of raw values, ratios, absorbance spectra, kinetic curves, or even color coded pictorials.
The new proprietary developed evaluation software allows displaying and analyzing endpoint, kinetic and spectral data using comprehensive data reduction algorithms and various curve fitting options. Up to 384 complete spectra or kinetic curves can be viewed individually or overlaid. For further data processing results can be easily exported to Microsoft Excel™.
