Coy Lab

Coy Lab Products

Coy Lab Products

For more than 30 years Coy Labs has produced Glove Boxes, Anaerobic Chambers, and controlled atmosphere glove boxes designed to fit a wide variety of needs. Make your selection from below to learn more about the quality product proudly produced by Coy Laboratory Products.

Basic Glove Boxes

Basic Glove Boxes

Basic Glove Boxes

Vinyl Glove Box

FLEXIBLE VINYL CHAMBERS These Flexible Glove Boxes are a simple low cost alternative to rigid glove boxes for sealed environments that can be purged with any inert gas through the standard Ball Valve and Pressure Relief Valve.

PVC CONSTRUCTION The glove boxes are constructed of heavy duty, flexible PVC mounted on a rigid base. Thickness ranges from 20 to 40 mils.

RELIABLE AND DURABLE All seams are radio frequency welded to produce a one piece bag that will withstand many years of service.

MANY APPLICATIONS In addition to medical laboratory applications, Coy controlled environment glove boxes have found other applications in industry due to their design and economy. These include micro welding, handling of hazardous materials, dry atmosphere, optical and electronic equipment assembly.

REDUCES OPERATOR FATIGUE The optically clear vinyl greatly increases operator vision, and the gloves do not fight back when entered, as in rigid glove boxes. In addition, there are no rigid ports to interfere with and consequently tire the operator’s arms.

MODULAR DESIGN Coy glove boxes are designed to be expandable and easily modified through the many options available.

CUSTOM SIZES AND CONFIGURATIONS Special size glove boxes are available at minimal additional cost, and accessories are available to economically create special configurations.

Vinyl Glove Box

Basic Glove Boxes
Aluminum Glove Box

Affordable, modular, Aluminum Glove Boxes provide isolation, containment, humidity, temperature, oxygen control, and anaerobic (complete removal of oxygen) atmospheres. These epoxy-coated, aluminum glove boxes are designed to your individual needs by selecting components from numerous interchangeable COY parts and accessories.Available in 1 and 2 Person Sizes (182 cm and 91 cm). 24″ depth (61 cm) and 36″ height (91 cm)

  • All construction is from the following durable materials:
  • Epoxy-coated aluminum (1/8″, 3.2mm thick),
  • Clear polycarbonate  (1/4″, 6.4mm thick),
  • Optically clear flexible PVC, polyvinyl chloride,
    (20-30 mils, 0.5-0.8mm thick)
  • EPOM rubber gaskets
  • Advanced silicone adhesives and sealants
  • Leak proof self sealing fasteners.

To maximize interior work area and ease of maintenance, many accessories are mounted to the exterior of the glove box.

Quick disconnect fittings have been used wherever possible.

Many of the accessories can be used together to simultaneously control multiple aspects of the glove box environment, such as humidity and oxygen control, or temperature and humidity.

The viewing screen is designed to provide good interior shadow-free ambient light.  Tempered Glass may be substituted for the standard polycarbonate for certain chemical operations.

All units are hermitically sealed and pressure checked at the factory for leaks. An inexpensive gas leak detector can be purchased for field testing.

GFI power lines are installed in humidified systems.

A Pressure Relief Valve and Ball Valve are installed on all units to provide a simple Flow thru gas system without over pressurizing the Glove Box.  For Isolation Systems the Pressure Relief Valve that vents to room air can be removed or sealed and equipped with tubing for routing to a fume hood or house evacuation system.

Also standard is the Patented Diaphragm Top for easy of operation and cost savings under anaerobic conditions.

 Aluminum Glove Box

Basic Glove Boxes
Polymer Glove Box

These glove boxes are an economical way to perform and maintain a wide range of atmospheres and functions, including use as a dry glove box, inert gas (nitrogen) glove box, isolation glove box, anaerobic chambers, oxygen, and humidity control glove boxes. Used with a variety of accessories and available in polycarbonate or UV resistant plastic, this glove box is easy and affordably customized for your needs.

 

Basic Glove Boxes

Containment or Isolation Glove Boxes

This Isolation Glove Box is designed to place an added barrier between you and any suspected contaminants contained within an envelope or package. This containment glove box’s hermetically sealed interior and neoprene gloves provide the air tight atmosphere you desire when opening tainted mail, and the neoprene gloves provide the greatest dexterity available while keeping your skin safe from contamination.The 18″ x 18″ side door of the glove box allows for larger packages to be passed into the glove box before being opened. Finally the patented COY Diaphragm Top allows the user to comfortably enter the chamber through the glove ports without fighting the gloves, and with out expelling the interior atmosphere to the room air through a bubblier or pressure relief valve. The COY Isolation Glove Box for anthrax containment is the peace of mind you and your employees have been looking for.

Anaerobic Chambers

Anaerobic Chambers

Anaerobic Chambers
Vinyl Anaerobic Chambers

DESIGN THEORYThe Vinyl Anaerobic Chambers provide a strict anaerobic atmosphere of 0-5 parts per million (ppm) using a palladium catalyst and hydrogen gas mix of 5%. The heavy duty vacuum airlock allows sample transfer without changes to the internal atmosphere. The Programmable airlock allows the user to tailor an interchange sequence ideal for their lab experiments.The Flexible nature of the unit allows for the user to reach deeper and higher into the unit. And the flexible nature of the unit combined with padded base and larger glove ports allows for greater ergonomics vs. a rigid glove box. The optically clear vinyl greatly increases operator vision, and the user does not encounter any glove “fight back” as the flexible nature allows the entire glove box to give with changes to internal volume.PVC CONSTRUCTION The glove boxes are constructed of heavy duty, flexible PVC mounted on a rigid base. Thickness ranges from 20 to 40 mils.RELIABLE AND DURABLE All seams are radio frequency welded to produce a one piece bag that will withstand many years of service.AIRLOCK A single push button begins the automatic sequencing of oxygen reduction within the airlock. Airlock can be adjusted to optimize the settings for the operation of the lab from experiment to experiment including number of cycles to level of the vacuum.ECONOMICAL Not only are Coy vinyl glove boxes less expensive than many comparable rigid glove boxes, but gas requirements are lower, the flexible glove box does not require any pressure relief or vent for excess gas keeping operating costs about 1/3 less than a rigid glove box of comparable size.MODULAR DESIGN Coy glove boxes are designed to be expandable and easily modified through the many options available.CUSTOM SIZES AND CONFIGURATIONS are available at minimal additional cost, and accessories are available to economically create special configurations.

 

Anaerobic Chambers
Gloveless Anaerobic Chambers

Gloveless Anaerobic Chambers offer the operator a convenient glove-free ability to handle and inspect samples under low oxygen (0-5 ppm) conditions. A special cuff and sleeve-vacuum system allows the operator to work barehanded (or with surgical gloves) inside the glove box. After the operator inserts their arms into the gloveless sleeves, a foot operated sleeve-vacuum system creates an inert atmosphere in the sleeves prior to removing the Arm Port Plugs. This permits the operator’s arms to enter the glove box without compromising the anaerobic chamber atmosphere. Tactile efficiency is improved. The Arm Port Plugs are used to seal the glove box while it is idle.Units operate similar to other COY anaerobic chambers using a palladium catalyst to remove O2 through a H2 Gas Mix (5%). Equipped with an airlock to establish low O2 conditions prior to transferring samples and instrumentation in/out of the work area.All COY Polymer Gloveless Anaerobic Chamber include a built in heating system (up to 40° C) and Glove Box Dehumidifier to maintain proper moisture levels through automatic digital control.From the two basic glove box styles, choose the design which suits your laboratory volume. Select either the compact, all clear, polycarbonate style with automatic purge airlock, or the larger aluminum glove box available with an automatic vacuum airlock.
 Gloveless Anaerobic Chambers

Anaerobic Chambers
Rigid Anaerobic Chambers (Gloved Units)

Rigid Anaerobic units can be manufacture from economical Polymer or the sturdy Aluminum.The Aluminum units are ideal for labs with chemical resistance needs, due to the ability to upgrade the standard polycarbonate viewing screen to a Tempered Glass, and the Glove Port Panel to an Aluminum style. Aluminum Anaerobic Chambers also have the advantage to upgrade to a gloveless style.The Polymer units are an easy and economical way to get started with an Anaerobic Chamber. Equipped with a purge only airlock these units are typically half the cost of the other style Anaerobic Chambers. It is important to keep in mind that while less expensive to purchase it may cost more to operate due to the purge only application of the airlock, but in a low volume lab this may be just the unit for you.Anaerobic Atmosphere The COY Anaerobic Chambers provide a strict anaerobic atmosphere of 0-5 parts per million (ppm) using a palladium catalyst and hydrogen gas mix of 5%. Air is circulated through a Fan Box which is designed to house the wire mesh container of catalyst called a Stak-Pak. The process of removing O2 from the atmosphere can produce moisture so often times these Stak-Paks are filled with desiccant and stacked on top of the Catalyst. The airlock allows sample transfer without changes to the internal atmosphere by equilibrating the O2 levels through a Vacuum and Purge Procedure (Purge only for the Polymer Units).Patented Diaphragm Top All units come equipped with this feature that allows the chamber to physically adjust to the users hands entering and exiting the glove box. No waiting for expensive electronics to compensate so there is no glove fight backGloves The Sleeves are manufactured from a durable neoprene with a separate cuff that allows the attachment/replacement of and inexpensive glove within minutes.

Humidity Control Chambers

Humidity Control Chambers

Humidity Control Chambers
Full Humidity Control

These units allow the user to control interior humidity levels above or below ambient conditions. All units are equipped with the latest in Humidity Sensor technology and Ultra Sonic Humidifier and either Desiccant Drying System or a Dry Gas Purge System.STYLES Two standard styles available, a clear Polycarbonate unit that features a lower cost to purchase, and relatively low cost to customize. The Aluminum units while more expensive on initial purchase are a longer lasting, sturdy build that with the addition of tempered Glass can be used in highly corrosive chemical use. Polycarbonate units have 4 standard sizes and Aluminum units have two.AUTOMATIC CONTROL The automatic controller provides monitoring and control of the relative humidity. It activates the necessary system (dehumidification for humidification) when the moisture level deviates from the adjustable set point. Flow rates, sensor position circulation, and orifice positions were all considered in the design to maximize control capabilities and humidity uniformity across the glove box. Since this is a closed loop circulation system, an inert gas environment can be maintained in the glove box while controlling the humidity.HUMIDIFIER The Ultra Sonic Humidifier vibrates the water in the reservoir to provide a cloud of mist that is pushed into the glove box from the pump system. At the same time air is pulled out of the glove box and circulated through the water reservoir, completing the Humidification System’s closed loop circuit. Water reservoir is equiped with Self Sealing Quick Disconnect inlet for refilling under sealed conditions.DRYING SYSTEMS Two systems are available to dry these Humidity Control Glove Boxes a Desiccant Drying System (Pictured above) and a Dry Gas Purge System. The Desiccant System is standard however the Dry Gas Purge System can be substituted at no extra cost. The Dry Gas Purge System allows for a lower attainable humidity level 3% (1% using special dry gas) vs 5% for the desiccant System. The Gas Purge System is also cleaner, provides faster drying times and requires less maintenance (Desiccant needs to be rejuvenated). The Desiccant Drying system can be a lot less expensive to operate. The two systems can also be used to work together to minimize the cost of the dry gas purge system.GLOVELESS SLEEVE All units are equipped with gloveless sleeves to allow superior handling of interior samples. However the moisture from skin does influence Humidity levels below 15% Rh and for these applications the user may need to operate with gloves which can easily placed over COY over the gloveless sleeve cuff. (Click here to see Gloveless Sleeves)TRANSFER CHAMBER OPTION All units have an optional pass thru chamber (air lock) available. This enables transfer of equipment and samples with out drastic changes to the interior environment.

Humidity Control Chambers
Dry Glove Boxes

Coy Dry Glove Boxes control and maintain humidity levels below ambient conditions, achieving a humidity level of 5% or lower through either a desiccant system for removing moisture or through a dry gas purge system, typically utilizing dry nitrogen or argon. Control is through a revolutionary sensor and monitor/controller providing precise control in .1% increments, with tuning features.STYLES Two standard styles are available: a clear Polycarbonate unit that features a lower cost to purchase, and relatively low cost to customize. The Aluminum units while more expensive on initial purchase are a longer lasting, sturdy build that with the addition of tempered Glass can be used in highly corrosive chemical use. Polycarbonate units have 4 standard sizes and Aluminum units have two.AUTOMATIC CONTROL The automatic controller provides monitoring and control of the relative humidity. It activates the necessary system (dehumidification for humidification) when the moisture level deviates from the adjustable set point. Flow rates, sensor position circulation, and orifice positions were all considered in the design to maximize control capabilities and humidity uniformity across the glove box. Since this is a closed loop circulation system, an inert gas environment can be maintained in the glove box while controlling the humidity.DRYING SYSTEMS Two systems are available to dry these Humidity Control Glove Boxes a Desiccant Drying System (Pictured above) and a Dry Gas Purge System. The Desiccant System is standard however the Dry Gas Purge System can be substituted at no extra cost. The Dry Gas Purge System allows for a lower attainable humidity level 3% (1% using special dry gas) vs 5% for the desiccant System. The Gas Purge System is also cleaner, provides faster drying times and requires less maintenance (Desiccant needs to be rejuvenated). The Desiccant Drying system can be a lot less expensive to operate. The two systems can also be used to work together to minimize the cost of the dry gas purge system.GLOVELESS SLEEVE All units are equipped with gloveless sleeves to allow superior handling of interior samples. However, the moisture from skin does influence Humidity levels below 15% Rh and for these applications the user may need to operate with gloves which can easily placed over COY over the gloveless sleeve cuff. Click here for more info on Gloveless Sleeves.

TRANSFER CHAMBER OPTION All units have an optional pass thru chamber (air lock) available. This enables transfer of equipment and samples with out drastic changes to the interior environment.

O2 Control Glove Boxes and Cabinets

O2 Control Glove Boxes and Cabinets
Extensive offering for InVivo and InVitro studies

The importance oxygen levels play in various biological processes is increasingly significant to researchers. To study this requires consistent atmospheric control — maintaining hypoxia, normoxia, hyperoxia, or anoxia to mimic physiological conditions. Coy offers a complete line of glove boxes and cabinets that enable you to maintain desired levels of CO2, temperature and humidity, along with oxygen control.Our modular design allows us to configure systems, including custom designs, to meet your needs whether for tissue culture or live animal studies.

Making Choices

Configure a system based on your application. Live animal or tissue culture systems are available that accommodate common accessories for these protocols. If you need to do manipulation within the controlled environment, glove boxes would be the best choice. Cabinets are most often used for short experiments or initial feasibility studies.

Glove Boxes utilize glove ports and an airlock (transfer chamber) to allow access to the chamber interior without disruption of the experimental environment, unlike cabinet or incubator systems.

Cabinets are simple hermetically sealed containers with shelving and a front-loading door, typically used for short-term experiments, dynamic oxygen-cycling studies, placement within standard incubators, or multi-level oxygen studies.

O2 Tissue Culture Glove Boxes typically consist of controls for oxygen, with CO2, temperature and humidity options. Think of these glove boxes as your incubator with gloves. Opening an incubator or working in a tissue culture hood exposes cells to non experimental conditions that may affect your results. Remove this experimental uncertainty by performing incubation, culture maintenance, and analysis in the same glove box environment.

Saturated humidity is maintained in a separate humidified box located inside the glove box. An internal dehumidifier maintains the glove box at non condensing moisture levels allowing instrumentation, such as microscopes, to be used inside the glove box.

Our InVitro cabinets are an excellent solution if you need to control multiple simultaneous O2 levels or desire to add O2 control to your existing CO2incubator.

O2 Animal Study Glove Boxes allow access to cages and the ability to change food and water quickly and easily while maintaining desired O2levels. Options include airlocks custom-sized to your cages, humidity control, and gaseous waste removal.

Our InVivo cabinets allow short-term studies where waste removal is not critical. They can be used for longer-term studies when disruption of the controlled environment for animal care does not cause an issue.

PCR Workstation

PCR Workstation

Products

CleanSpot PCR Workstation

The CleanSpot is designed to fulfill a very important need today… control of PCR* contamination.Just 25 – 30 minutes of UV irradiation in the Coy Clean Spot can prevent unwanted PCR contamination. The Clean Spot is the easiest and most economical method for DNA (RNA) purification.The combination of a special light reflector and two powerful overhead UV bulbs generate Pyrimidine Dimers (T,C) and other photo defects in contaminating target sequences, thus eliminating falsely primed products that result in countless lost time and expense.The CleanSpot is designed to prevent unwanted contaminant DNA in amplification mixtures, thereby saving many lost hours and expensive samples.The UV resistant acrylic enclosure provides a contained, dead air space in which to set up your reactions and permit safe 254nm UV irradiation of solutions and supplies. This powerful UV wavelength renders DNA contaminants biologically inactive in 30 minutes or less.The thick front panel is an effective beta irradiation shield as well as acting as a breath guard. An overhead fluorescent light illuminates the work area.DETAILED DESCRIPTIONDNA sequences often contaminate reagents, buffers, equipment, tubes and tips commonly used for PCR* reactions, resulting in needless lost time and expense.For years it has been known that UV light can damage DNA. UV exposure of 30 minutes or less in the Cleanspot PCR Work Station can prevent unwanted amplification of contaminant DNA. The combination of unique reflectors and powerful overhead UV lights introduces pyrimidine dimers and other photo damage into the contaminating target sequences, making them non-amplifiable.

The amplification process produces large quantities of DNA that are handled at various analysis stations, and even laboratories using extreme care in handling the samples will have the product permeate through the lab. One copy of this product DNA or other extraneous DNA in an experimental reaction mixture can be amplified, leading to false positives or erroneous results. This contaminant DNA is typically introduced during reaction set up, being carried into the mixture by:

  1. Contaminated tubes, tips, pipettes;
  2. Sirborne particles laden with DNA;
  3. Fouled reaction components.

The PCR Workstation is designed to combat the three major contaminant pathways. Reaction preparation and equipment/supply storage is performed in the “clean,” enclosed, still air chamber, thus minimizing airborne and supply borne contamination. During reaction preparation, part of the reaction mix is exposed to the UV light, thus decontaminating any fouled reaction components. Since this exposure bathes the chamber and its contents, they are “cleaned” at the same time. By keeping the doors latched, the enclosed chamber and supplies are ready for the next use.

Multiple Item Irradiation
Large enclosed work area permits continuous irradiation of many items simultaneously.

More Effective Irradiation of Reaction Mixtures, Reagents and Supplies
Unique light reflector allows all UV light to be directed to the work area, providing more effective irradiation of reaction mixtures, reagents, pipette tips, and pipettors.

Reduced Operator Exposure to UV
Safety device switches off the lamps preventing user exposure inside chamber. Acrylic absorbs UV light for added safety.

Eliminates Falsely Primed Products in 30 minutes or less
The shelf can be used as an exposure area to reduce decontamination time.

White Light Illumination
Overhead white light illuminates the work area for non-UV light applications.

(32)P Workstation, Ideal for Cycle Sequencing Preparation
Thick 3/8 inch acrylic front panel is an effective beta shield, providing protection from beta radiation emitted from (32)P.

Isolating Environment
The enclosed benchtop work area provides a convenient, “clean,” still air environment in which to set up reactions.

Easy to Install and Clean
The chamber breaks down into 4 component parts without the use of tools.

Advantages Over Other Methods
In addition to enjoying cost advantages over laminar hoods, the CleanSpot can have performance benefits due to the enclosed work area and UV lights. The amplified DNA is not altered, as with chemical decontamination methods. An important facet in preventing contamination, the isolated, “clean” set up area is missing when trans-illuminators or crosslinkers are used as radiation sources. Also, these light sources may not emit light at a high enough intensity or at the proper wavelength to efficiently inactivate the contaminant DNA.

PERFORMANCE INFORMATION

A. Pure Target
100% of sequences can be amplified. 10 ng of a previous PCR product resuspended in 4ul of distilled H2O were allocated into a series of tubes. All tubes were run through UV light irradiation and 1 tube was removed every 10 minutes. Each tube was then cycled through 30 rounds of PCR using primers specific to the material PCR and 10ul of each reaction was run on an agarose gel. Tubes were on their sides and capped.

B. Human Genomic DNA Target
1. Repetitive sequence DNA primers were used on total genomic DNA which was irradiated over a time course of 10 to 30 minutes and each tube was cycled through 30 rounds of PCR using repetitive DNA primers. 10ul of amplified DNA was run on a agarose gel. 20 minutes of irradiation was required to deactivate 20ng of target. Tubes were on their sides and capped.

2. 50 ng of total human DNA resuspended in 4ul of distilled H2O was irradiated over a time course of 10 to 30 minutes. Each tube was cycled through 30 rounds of PCR using a primer set specific for a single gene. 20 minutes completely deactivated the template.

The irradiation times given in A and B above can be reduced by placing the items to be decontaminated on the shelf in the chamber. Since the light is about two times more intense on the shelf, the time required to deliver the same amount of energy to the items is 1/2 that at the chamber base where tests A and B were done.

Suggested irradiation times take into account the intensity given off by the UV lights and the natural decay in this intensity over time. A decrease of 20% in intensity is seen after 1500 hours of continuous operation. Since this decay is accelerated by switching the bulb on and off. we suggest changing the bulbs every year.

Current users are finding the CleanSpot very useful in eliminating contamination problems in labs where amplification of the same gene has been done for a long time.

 

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