PHERAstar — High-End Microplate Reader
PHERAstar, the premier multi-detection HTS microplate reader. No matter what reading technology you use in your laboratory, the PHERAstar will provide you with the best sensitivity available, and the fastest reading speed on the market.
The compact and versatile PHERAstar microplate reader is able to perform all leading non-isotopic detection technologies including:
- Fluorescence Intensity - including FRET with simultaneous dual reading
- Fluorescence Polarization
- Time-Resolved Fluorescence - including DELFIA™
- TR-FRET - including HTRF®, LanthaScreen™, LANCE™
- High - end AlphaScreen™ - now available with enhanced laser light excitation
- Luminescence - including BRET with simultaneous dual reading
- Absorbance UV/Vis
The PHERAstar is the ONLY microplate reader on the market with five photomultiplier tubes (PMTs) optimized for specific reading modes. This ensures that your assay is read with highest sensitivity a feature that no other microplate reader offers. The PMTs are automatically selected for your reading mode and require absolutely no intervention from the user.
Due to the unique modular optical system, sequential dual excitation, simultaneous dual emission, and ratiometric calculations such as FRET and BRET are all performed with ease and speed. The barcoded Optic Modules have the appropriate filters, mirrors, beamspliter, dichroics or polarizers incorporated into one hand-held module. These are easily inserted into the reader by hand. The PHERAstar will then automatically select the proper module for your assay protocol. Never worry about what dichroic, aperture, or filter is installed in your microplate reader again.
The PHERAstar provides superior sensitivity, speed and dynamic range. Not only does it support endpoint readings, but is also capable of kinetic measurements and well scanning with better control and flexibility than competing readers.
- Real-time display - get kinetic curves as the data is collected. View as raw data, graphs, color pictorials - all in real time as the machine is running
- Well scanning - up to 10 x 10 matrix scans in wells with evaluator to allow you to select points or areas to use for quantitation
- TR-FRET decay - can collect TR-FRET curves with 2 µs resolution - in real time
- Read frequency per point from 20 ms to over three hours
Assay flexibility is further enhanced by precise temperature control to 45°C as well as multi-mode shaking capabilities. Both are standard on the PHERAstar. The PHERAstar can read 6-1536 well plates with uncompromised sensitivity, - no need for special apertures or optics.
With the PHERAstar, BMG LABTECH offers a unique combination of features to support all major existing applications as well as your future needs. Applications include:
- Protein-protein interactions
- Genotyping
- Molecular binding assays
- Receptor ligand binding
- DNA quantification
- Enzyme activity and kinetic assays
- Cell based assays
- Reporter gene assays
- Protein quantification
- G-coupled protein receptors
- Cell based assays
And many more assays
