GeneTools is a powerful image analysis program for all gel applications: 1D gels, spot and slot blots, plates, colonies, autorads, multi-tier gels, protein gels, GFP, PCR, Coomassie Blue and silver-stained gels, ZYMA gels, pattern matching, and more. GeneTools version 3 has a unique E-Gel® 96 module which allows one step analysis of E-Gel 96 gels in seconds, and can be purchased as an additional option.
Achieving input to results in under 8 seconds, GeneTools is fully automatic, easy to use and very fast.
GeneTools is available on every Syngene system and comes with free upgrades for life.
Features
| Feature |
Benefit |
| 'One Click' technology for a full, rapid and accurate analysis in seconds |
Very easy to use with minimal training |
| Auto track, band, peak, lane and edge finding even on smiling gels and grimaced bands |
Virtually 'hands free' operation |
| Auto and manual background subtraction with a range of methods |
Complete control of the analysis process |
| Numerous applications |
Very versatile |
| Multi-tier analysis for high throughput screening |
Saves time |
| Superior colony counting function with ability to count 2 colour colonies |
Extends applications into microbiology |
| Molecular weight libraries |
Build databank |
| Pattern matching analysis and dendrogram display |
For band matching applications within the same package |
| Integral image browser |
Multiple functions for all applications |
| Adherence to Good Laboratory Practice at every stage including reporting |
Maintains integrity of results |
| Direct exporting of results to Excel and Word |
Seamless operation with other packages |
GeneTools Functions
1D analysis
- Auto track finding - NO limit - using a single click
- Auto band finding
- Auto edge finding
- Manual override of all automated functions if required
- Auto baseline correction - 5 different methods including manual selection
- Track finding in ANY direction - down, up, left to right, right to left
- Multi-tier analysis for high throughput screening
- Peak detection parameters can be altered to suit user
- Unlimited track width setting
- Auto track, band and edge numbering and identification with user defined colours
- Track labelling
- Track histogram alongside image for easy identification
- Manual band addition and deletion with recording for GLP purposes
- Track profile analysis - unlimited track overlays
- Molecular weight calibration curve display
- Quantity calibration curve display
- User configurable results of: position, distance travelled, peak height, area, raw volume, % raw volume, Rf, MW, Quantity, calibration standard, peak width
Molecular weight
- Library of markers for automatic or manual assignment
- User configurable library
- Unlimited number of markers can be used per gel
- Auto interpolation of multiple markers for use with smiling or distorted gels
- Curve type, calibration method selection (log linear fit, log piecewise linear)
- Calibration curves are displayed
Quantity
- Manual input of calibrated standards and quantities
- Various linear or quadratic calibrations can be selected
Spot blot
- Optional spot or slot blot
- Works in grids or non grid formats
- Random spot/slots automatically located
- Auto detection of absorption or fluorescence
- Ability to set all spot/slots to one size or auto detection with variable size
- Auto compensation for grid variances
- Thresholding of spots
- Manual control of spot sizes, positions and grid position
- Background corrections – manual or auto
- No limit to the number of manual background subtractions
- Auto selection of nearest background for subtraction
- Incidence parameters for positive and negative reactions with both 'greater than' and 'less than' values or a range of values can be set
- Incidence value can be set using raw volume, percentage of total, pixel area of spot/slot, calibrated quantity value
- Volume calibrations
- Display of calibration curves
Colony counting
- Superb NEW colony counting function
- Auto counting of colonies with manual editing capability
- Ability to count light and dark colonies on the same plate as well as two colour colonies at any one time
- Ability to vary the position and size of area of interest
- Unique auto-separation of colonies
- Proprietary algorithm gives near 100% accuracy - therefore no need for an adding or subtraction, manual editing function
- Variable sensitivity for detection
- Ability to set min and max colony areas
- Class split for two colour colonies giving count and % area of plate covered
- Ability to show shape of detected colony
- Manual X and Y positions
- Brightness, contrast and Gamma control
- Define areas of exclusion – also adjustable
- Results table
Arrays and plates
- Total auto detection of spot patterns without the need for manual skewing
- Detection of spot patterns at any angle without need for adjustment
- Auto background subtraction, no need for a blank background subtraction
- Immediate export into Excel of all data for comparison information
Gel scoring
- Auto positive and negative and presence and absence of bands
- Results shown as table
Band matching
- GeneTools has band matching facilities. This gives the user the ability to align bands, determine track relationships and compare track data. This is all achieved automatically but with the added advantage of manual override.
- Band matching by position, defined Rf or calibrated molecular weight
- Tolerance variation (%)
- Auto match function
- Dendrogram, cluster analysis
- Matching comparisons (presence and absence)
- Matching matrix
- Matching coefficients
Manual band quantification
This section of GeneTools allows the user to manually quantify bands, spots or other objects on a sample. The drawing tool may be rectangular or free-hand, giving total flexibility. Once all areas of interest are specified, a background correction may be applied automatically or manually selected from the surrounding sample. The user is able to quantify and perform threshold analysis using positive and or negative controls. Standard curve data may also be entered. All data is instantly exportable to Excel ® .
