IEF-SYS high resolution 2D gel electrophoresis system
The IEF-SYS is a robust acrylic horizontal gel tank designed for running 2D electrophoresis gels with immobilized pH gradient (IPG) strips and pre-cast acrylamide gels. IPG strips, widely acknowledged as the best method of resolving proteins by their isoelectric point are laid on the IEF-SYS system's hollow frame. The position of positive and negative electrodes on the hollow frame can be adjusted to ensure optimum contact with the IPG strips' edges, making it easy to control the voltage gradient across the strip.
A ceramic cooling block filled with Hexid, the best super cooling fluid currently available is placed under the hollow frame, allowing users to run high voltage through the IPG strips, resulting in precisely focused protein spots. The IEF-SYS system's glass bottomed electrode frame is then used to run the second dimension molecular weight separation using the pre-run IPG strips with a pre-cast acrylamide gel to achieve perfect 2D gel results using just one tank.
Features
| Feature |
Benefit |
| Top frame options with variable electrode spacing systems |
Hollow frame for IEF, where the strips are placed directly onto the ceramic plate and glass bottomed frame for pre-cast horizontal gels |
| Two sets of electrodes supplied |
Detachable and tank mounted electrodes for ease of use |
| Large cooled ceramic top plate |
Provides uniform cooling of the gel platform |
| Buffer drainage ports |
Built-in shut-off valve is opened when the drainage tube is connected to empty the buffer chamber |
Specifications
| Component |
Specification |
| Gel tank |
Horizontal acrylic tank with built-in buffer drainage ports |
| Frames |
Hollow frame for IPG strips, glass bottomed frame for pre-cast acrylamide gels |
| Ceramic cooling block |
27 x 27cm sealed black with integral cooling coil and auto-shut off valves |
| Electrodes |
Tank mounted set for running wick gels, moveable set for running IPG strips or acrylamide gels |
| Power input |
100 - 240V |
| Dimensions |
460mm x 115mm |
| Depth |
430mm (tanks and cooling connectors), 340mm (tank only) |
| Buffer volume |
275ml |
